sRNA profiling in Aspergillus flavus reveals differentially expressed miRNA-like RNAs response to water activity and temperature.

  发布时间: 2016-03-25   信息员:   

sRNAprofiling in Aspergillus flavus reveals differentially expressed miRNA-like RNAs response to water activityand temperature.

Abstract

Small non-coding RNA (sRNA) in various organisms remains a mysterious subject. Although microRNAs (miRNAs) have been intensively investigated in plants and animals, the study of miRNAs in fungi has been limited. Only microRNA-like RNAs (milRNAs) have been reported in several filamentous fungi. In this study, Illumina deep sequencing was performed to characterize the sRNA in Aspergillusflavus and to evaluate their responses to wateractivity and temperature. Global expression analysis showed an extensively differential expression of sRNA loci in A. flavusunder different temperature or water activities. In addition, a total of 135 milRNAs were identified in A. flavus. The milRNA profiles obtained in deep sequencing were further validated by RT-qPCR assay. The presence and differential expression of milRNAs under different temperature or wateractivities in A. flavus imply that milRNAs might play important roles in the mycotoxin biosynthesis and mycelium growth in fungi A. flavus.

Citation

Bai Y, Lan F, Yang W, Zhang F, Yang K, Li Z, Gao P, Wang S (2015) sRNA profiling in Aspergillus flavus reveals differentially expressed miRNA-like RNAs response to water activity and temperature. Fungal genetics and biology 81:113-119


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