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海峡生命科学前沿论坛第一百零八讲(2018.11.7)-Regulation of light-induced stomatal opening and the plasma membrane H+-ATPase等


海峡联合研究院   发布时间: 2018-10-31  信息员:  

时       间:   2018117日,上午9:00-10:00

地       点:博学报告厅

主办单位:海峡联合研究院园艺中心

报  告  人:Tetsuya Higashiyama

Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University,

报告题目:Dynamics and key signalling molecules of pollen tube guidance

  

Academic career:

2013-(present), Vice Director, Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University 2007-present Professor, Nagoya University;

1999-2006 Research Associate, University of Tokyo;

1999 Ph.D. University of Tokyo;

Summary:

Successful sexual reproduction of flowering plants involves complex cell-to-cell communication. We have been working on pollen tube guidance, double fertilization, and early embryogenesis by live-cell analysis. In pollen tube guidance, defensin-like peptide LUREs are pollen tube attractants ofTorenia andArabidopsis secreted by two egg-accompanying “synergid cells” (Science 2001; Nature 2009;PLoS Biol. 2012). To understand the molecular mechanism of pollen tube guidance, we have been taking two approaches of live-cell study. The first approach is to use precisely defined semi-in vitro system (semi-in vivo system), including development of various microfluidics devices (e.g.,RSC Adv. 2013; Biomicrofluidics 2018). Semi-in vitro studies combined with synthetic chemistry lead to discovery of novel intercellular signaling molecules involved in pollen tube guidance. Arabinogalactan sugar chain AMOR derived fromTorenia ovular sporophytic tissues is critical to make pollen tubes competent before attraction by TfLUREs (Curr. Biol. 2016; Plant Physiol. 2017). PRK6 is a receptor kinase ofArabidopsis, which is critical in sensing of AtLURE1 peptides (Nature 2016). Cocrystal structure analysis of AtLURE1 and PRK6 suggested unique action mechanism of AtLURE1 (Nat. Commun. 2017). The second approach is based on in vivo imaging. We have shown that pollen tube guidance is intimately related with double fertilization (e.g., Dev. Cell 2013;Cell 2015). By using two-photon microscopy, we have succeeded in visualizing pollen tube guidance in the pistil tissue (e.g., Development 2015, Protoplasma 2015). In this talk, I will talk about recent progresses of our pollen tube guidance study to discuss how pollen tubes are navigated to ovules, focusing on precise directional control, competency control, species-specific attraction, and one-to-one relationship between multiple ovules and pollen tubes.

  

Publications:

Higashiyama T. et al. (2001)Science 293, 1480-1483; Okuda S. et al. (2009)Nature458, 357-361; Hamamura Y. et al. (2011)Curr. Biol. 21, 497-502; Takeuchi H. and Higashiyama T. (2012)PLoS Biol., e1001449; Maruyama D. (2015)Cell61, 907-918; Gooh K. (2015)Dev.Cell34, 242-251; Mizukami A.G. et al. (2016)Curr. Biol. 26, 1091-1097; Takeuchi H. and Higashiyama T. (2016)Nature 531, 245-248; Zhang X. et al. (2017)Nat. Commun. 8, 1331.

  

时        间:  2018117日,上午10:00-11:00

地        点:博学报告厅

主办单位:海峡联合研究院园艺中心

报   告 人:Toshinori Kinoshita

Institute of Transformative Bio-Molecules (ITbM), Nagoya University

报告题目:Regulation of light-induced stomatal opening and the plasma membrane

H+-ATPase

  

Academic career:

2013-presentProfessor, Institute of Transformative Bio-Molecules (ITbM),

Director, Center for Gene Research, Nagoya University

2010-presentProfessor, Graduate School of Science, Nagoya University

2007-2010 Associate Professor, Graduate School of Science, Nagoya University

2005-2009PRESTO Researcher, Japan Science and Technology Agency

2003-2004Visiting Scientist, Salk Institute (Prof. Joanne Chory)

1994-2007Research Associate, Assistant Professor, Kyushu University

  

Summary:

Stomata in the plant epidermis control gas exchange between plants and atmosphere. Stomatal aperture is regulated by many environmental signals, such as light, water status, temperature, and CO2. Under blue light, plasma membrane (PM) H+-ATPase in stomatal guard cells is activated via blue light-receptor phototropins and signaling mediators, such as BLUS1, BHP, and PP1. Blue light-activated PM H+-ATPase provides driving force for stomatal opening. However, details of the signaling between phototropins and PM H+-ATPase still remain unclear. Therefore, we are trying to clarify this signaling pathway by several methods. In addition, based on the results from these basic researches, we are trying to control stomatal aperture by genetic and chemical approaches, and found that enhancement of light-induced stomatal opening by overexpressing PM H+-ATPase in guard cells increases photosynthesis and plant growth, and that suppression of stomatal opening by chemicals confers drought tolerance on plants. These results clearly indicate that manipulation of stomatal aperture is very useful technique for controlling plant growth and survive.

PM H+-ATPase has critical roles not only in stomatal opening, but also in cell expansion, nutrient uptake, maintenance of membrane potential, and so on. Recently, we found that PM H+-ATPase activity is regulated by plant hormones, auxin and brassinosteroid, in the etiolated seedlings and photosynthesis in mesophyll cells. I also will talk about the regulation of PM H+-ATPase by these signals.

  

Publications:

Toh et al. (2018)Plant Cell Physiol,59, 1568-1580., Uchida et al. (2018)Nat Chem Biol,14, 299-305.,Hayashi et al. (2017)Sci Rep, 7, 45586., Inoue and Kinoshita (2017)Plant Physiol,174, 531-538.,Okumura et al. (2016)Plant Physiol,171, 580-589.,Wang et al. (2014)PNAS,111, 533-538., Takahashi et al. (2012)Plant Physiol, Okumura et al. (2012)Plant Physiol, 159, 826-834., Kinoshita et al. (2011)Curr Biol, 21, 1232-1238., Kinoshita et al. (2001)Nature, 414, 656-660.